一种高压光学样品池系统的设计与实验研究

朱瑞滨; 刘伟龙; 宋云飞; 王伟; 张福长; 罗利军; 王文田; 贾培起; 刘晓旸; 杨延强; 苏文辉

doi:10.11858/gywlxb.2012.05.001

一种高压光学样品池系统的设计与实验研究

doi: 10.11858/gywlxb.2012.05.001

1.
哈尔滨工业大学凝聚态科技中心，黑龙江哈尔滨 150080；
2.
天津华泰森淼生物工程技术有限公司，天津 300384；
3.
吉林大学无机材料合成与制备化学国家重点实验室，吉林长春 130012

详细信息

通讯作者:
王伟 E-mail:wwang_ol@hit.edu.cn

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出版历程
- 收稿日期: 2011-02-17
- 修回日期: 2011-06-15
- 发布日期: 2012-10-15

An Instrument Design of High-Pressure Optical Cell and Its Implication in Experimental Research

1.
CCMST, Harbin Institute of Technology, Harbin 150080, China;
2.
Tianjin Huataisenmiao Biotechnology Co. Ltd, Tianjin 300384, China;
3.
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, China

摘要

摘要: 设计和研制了一种用于溶液样品光学特性原位检测的高压光学样品池成套系统，组件包括手动泵、增压器和高压样品池。其中样品池开有3处光学窗口，以水为传压介质，设计的最高使用压力为600 MPa。当用JGS1石英作窗口时，密封和保压效果良好；由于受到石英材质强度的限制，使用压力应控制在300 MPa以下。为检验该系统在高压原位检测方面应用的可行性，进行了色氨酸高压荧光光谱的采集。
- 高压光学样品池 /
- 原位检测 /
- 色氨酸 /
- 荧光
Abstract: An instrument design of a high-pressure optical cell system for in situ detection of aqueous samples was reported, including a manual pump, a pressure multiplier, and a high-pressure cell. The cell with three optical windows can be used within a pressure range of up to 600 MPa, using water as the pressure transmitting medium. When JGS1 quartz windows were employed, the safe pressure was around 300 MPa. The hermetic sealing effect of quartz was so good that the pressure was hold very well during experiments. In order to demonstrate the functioning of the system, the fluorescence spectra of tryptophane was determined at different pressures.
- high-pressure optical cell /
- in situ detection /
- tryptophane /
- fluorescence

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参考文献(41)

Mao W L, Mao H, Meng Y, et al. X-ray induced dissociation of H2O and formation of an O2-H2 alloy at high pressure [J]. Science, 2006, 314: 636-638.

Ma Y, Eremets M, Oganov A R, et al. Transparent dense sodium [J]. Nature, 2009, 458: 182-185.

Graham R A, Morosin B, Venturini E L, et al. Materials modification and synthesis under high pressure shock compression [J]. Annu Rev Mater Sci, 1986, 16: 315-341.

Jin C Q, Zhou J S, Goodenough J B, et al. High-pressure synthesis of the cubic perovskite BaRuO3 and evolution of ferromagnetism in ARuO3 (A=Ca, Sr, Ba) ruthenates [J]. Proc Natl Acad Sci USA, 2008, 105: 7115-7119.

Spandler C, Hermann J. High-pressure veins in eclogite from New Caledonia and their significance for fluid migration in subduction zones [J]. Lithos, 2006, 89: 135-153.

Su W H, Liu S E, Xu D P, et al. Effects of local mechanical collision with shear stress on the phase transformation from alpha-quartz to coesite induced by high static pressure [J]. Phys Rev B, 2006, 73: 144110.

Jenner G. Comparative activation modes in organic synthesis. The specific role of high pressure [J]. Tetrahedron, 2002, 58: 5185-5202.

Hemley R J. Effects of high pressure on molecules [J]. Annu Rev Phys Chem, 2000, 51: 763-800.

Schettino V, Bini R. Molecules under extreme conditions: Chemical reactions at high pressure [J]. Phys Chem Chem Phys, 2003, 5: 1951-1965.

Krzyzaniak A, Salański P, Jurczak J, et al. B-Z DNA reversible conformation changes effected by high pressure [J]. FEBS Lett, 1991, 279: 1-4.

Lullien-Pellerin V, Popineau Y, Meersman F, et al. Reversible changes of the wheat 46 gliadin conformation submitted to high pressures and temperatures [J]. Eur J Biochem, 2001, 268: 5705-5712.

Jackson A J, McGilivray D J. Protein aggregate structure under high pressure [J]. Chem Commun, 2011, 47: 487-489.

Li Y G, Su L, Li S P, et al. Effects of high pressure treated seeds on the growth and development of watermelon [J]. Chinese Journal of High Pressure Physics, 2010, 24(2): 143-153. (in Chinese)

李玉阁, 苏磊, 李锁平, 等. 高压处理种子对西瓜生长发育的影响 [J]. 高压物理学报, 2010, 24(2): 143-153.

Kovac K, Diez-Valcarce M, Hernandez M, et al. High hydrostatic pressure as emergent technology for the elimination of foodborne viruses [J]. Trend Food Sci Technol, 2010, 21: 558-568.

Akasaka K. High pressure NMR study of proteins-seeking roots for function, evolution, diseases and food applications [J]. High Press Res, 2010, 30: 453-457.

Taniguchi Y, Takeda N, Ado K, et al. High pressure FT-IR spectroscopic study on the secondary structure changes in insulin amyloid fibril and aggregate [J]. High Press Res, 2009, 29: 676-679.

Goncharov A F, Struzhkin V V. Raman spectroscopy of metals, high temperature superconductors and related materials under high pressure [J]. J Raman Spectrosc, 2003, 34: 532-548.

Lowwitzer S, Winkler B, Tucker M. Thermoelastic behavior of graphite from in situ high-pressure high-temperature neutron diffraction [J]. Phys Rev B, 2006, 73: 214115.

Li F F, Cui Q L, Cui T, et al. In situ Brillouin scattering study of water in high pressure and high temperature conditions [J]. J Phys: Condens Matter, 2007, 19: 425205.

Han Y H, Luo J F, Gao C X, et al. Phase transition of graphitic C3N4 under high pressure by in situ resistance measurement in a diamond anvil cell [J]. Chin Phys Lett, 2005, 22: 1347-1349.

Liu H, Wang L, Xiao X, et al. Anomalous high-pressure behavior of amorphous selenium from synchrotron X-ray diffraction and microtomography [J]. Proc Nat Acad Sci USA, 2008, 105: 13229-13234.

Zheng H F, Sun Q, Zhao J, et al. Comment on the pressure gauge for the experiments at high temperature and high pressure with DAC [J]. Chinese Journal of High Pressure Physics, 2004, 18(1): 78-82. (in Chinese)

郑海飞, 孙樯, 赵金, 等. 金刚石压腔高温高压实验的压力标定方法及其现状 [J]. 高压物理学报, 2004, 18(1): 78-82.

Paladini A A, Weber G. Absolute measurements of fluorescence polarization at high pressures [J]. Rev Sci Instrum, 1981, 52: 419-427.

Johnson M R, Burgmair M, Trommsdorff H P. A high hydrostatic pressure cell for optical experiments at low temperatures [J]. Rev Sci Instrum, 1995, 66: 3551-3554.

Addleman R S, Hills J W, Wai C M. A simple high pressure flow cell for on-line absorption, Raman, and time resolved laser induced fluorescence spectroscopy in supercritical fluids [J]. Rev Sci Instrum, 1998, 69: 3127-3131.

Zhou J G, Koulas S, Chong P L. Shape memory alloy activated high-pressure optical cell for biophysical studies [J]. Rev Sci Instrum, 2000, 71: 4249-4256.

Woenckhaus J, Khling R, Winter R, et al. High pressure-jump apparatus for kinetic studies of protein folding reactions using the small-angle synchrotron X-ray scattering technique [J]. Rev Sci Instrum, 2000, 71: 3895-3899.

Zahl A, Igel P, Weller M, et al. Compact high pressure unit for ultraviolet-visible-near-infrared spectroscopic measurements at pressures up to 400 MPa [J]. Rev Sci Instrum, 2003, 74: 3758-3762.

Frey B, Hartmann M, Herrmann M, et al. Microscopy under pressure-An optical chamber system for fluorescence microscopic analysis of living cells under high hydrostatic pressure [J]. Microsc Res Tech, 2006, 69: 65-72.

Kohlbrencher J, Bollhalder A, Vavrin R, et al. A high pressure cell for small angle neutron scattering up to 500 MPa in combination with light scattering to investigate liquid samples [J]. Rev Sci Instrum, 2007, 78: 125101.

Ando N, Chenevier P, Novak M, et al. High hydrostatic pressure small-angle X-ray scattering cell for protein solution studies featuring diamond windows and disposable sample cells [J]. J Appl Crystallogr, 2008, 41: 167-175.

Hoshihara Y, Kimura Y, Matsumoto M, et al. An optical high-pressure cell for transient grating measurements of biological substance with a high reproducibility [J]. Rev Sci Instrum, 2008, 79: 034101.

Meier G, Kriegs H. A high pressure cell for dynamic light scattering up to 2 kbars with conservation of plane of polarization [J]. Rev Sci Instrum, 2008, 79: 013102.

Shao G H, Wei Z C. Ultrahigh Pressure Vessel [M]. Beijing: Chemical Industry Press, 2002: 124-134.

邵国华, 魏兆灿. 超高压容器 [M]. 北京: 化学工业出版社, 2002: 124-134.

Lange R, Balny C. UV-visible derivative spectroscopy under high pressure [J]. Biochim Biophys Acta, 2002, 1595: 80-93.

Cioni P, Strambini G B. Tryptophan phosphorescence and pressure effects on protein structure [J]. Biochim Biophys Acta, 2002, 1595: 116-130.

Font J, Torrent J, Ribo M, et al. Pressure-jump-induced kinetics reveals a hydration dependent folding/unfolding mechanism of ribonuclease A [J]. Biophys J, 2006, 91: 2264-2274.

Ruan K, Tian S, Lange R, et al. Pressure effects on tryptophan and its derivatives [J]. Biochim Biophys Res Commun, 2000, 269: 681-686.

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