热压协同处理对接种于鸡腿菇中的细菌芽孢灭活效果的影响

王标诗; 李汴生; 曾庆孝; 黄娟; 阮征; 李琳

doi:10.11858/gywlxb.2010.02.010

热压协同处理对接种于鸡腿菇中的细菌芽孢灭活效果的影响

doi: 10.11858/gywlxb.2010.02.010

1.
湛江师范学院化学科学与技术学院，广东湛江 524048；
2.
华南理工大学轻工与食品学院，广东广州 510640

详细信息

通讯作者:
王标诗

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出版历程
- 收稿日期: 2009-11-11
- 修回日期: 2009-12-12
- 发布日期: 2010-04-15

Inactivation Effects of Coprinus Comatus Inoculated with Bacterial Spores by High-Pressure Thermal Processing

1.
School of Chemistry Science and Technology, Zhanjiang Normal College, Zhanjiang 524048, China;
2.
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China

More Information

Corresponding author: WANG Biao-Shi

摘要

摘要: 以鸡腿菇为原料，以凝结芽孢杆菌芽孢和嗜热脂肪芽孢杆菌芽孢为对象菌，研究中温与超高静压协同处理条件下两种对象菌的灭活效果及其相应的动力学变化趋势。研究结果表明：在相同条件下，鸡腿菇中凝结芽孢杆菌芽孢的致死效果优于嗜热脂肪芽孢杆菌；接种于鸡腿菇中的两种对象菌芽孢致死效果均低于磷酸缓冲液中的效果，说明鸡腿菇对这两种细菌芽孢有一定的保护作用。利用线性模型、Weibull模型和Log-logistic模型，对鸡腿菇中两种对象菌芽孢的灭活效果进行拟合，以均方差和回归系数为评价指标，分析了3种模型的拟合效果。结果表明：在所研究的条件下，Log-logistic模型的拟合效果最好，Weibull模型次之，线性模型最差。因此可以通过Log-logistic模型来预测热压协同处理下细菌芽孢的灭活效果。
- 超高静压 /
- 中温 /
- 鸡腿菇 /
- 灭活 /
- 凝结芽孢杆菌芽孢 /
- 嗜热脂肪芽孢杆菌芽孢
Abstract: The combination effect of high hydrostatic pressure and moderate heat on the inactivation effects of coprinus comatus inoculated with bacterial spores was investigated. The results showed that two bacterial spores (Bacillus coagulans and Bacillus stearothermophilus) had a much higher resistance in coprinus comatus than in phosphate buffer solution. Moreover, Bacillus stearothermophilus spore had a much higher resistance than Bacillus coagulans spore at the same pressure and temperature. Survival data were modeled using the linear, Weibull and Log-logistic models to obtain relevant kinetic parameters. The mean square error and regression coefficient suggested that the Log-logistic model produced best fits to all survival curves, following by Weibull model, and the linear model was not adequate for describing these curves. The Log-logistic model could be used to forecast the inactivation curves of bacterial spores by the combination of high hydrostatic pressure and moderate heat.
- high hydrostatic pressure /
- moderate heat /
- coprinus comatus /
- inactivation /
- Bacillus coagulans spore /
- Bacillus stearothermophilus spore

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参考文献(26)

Li B S, Ruan Z. Nonthermal Sterilization Technology and Application [M]. Beijing: Chemical Industry Press, 2004: 121. (in Chinese)

李汴生, 阮征. 非热杀菌技术及应用 [M]. 北京: 化学工业出版社, 2004: 121.

Wang B S, Zeng Q X, Li B S, et al. Research Advances in Thermal Effect in High Hydrostatic Pressure (HHP) Processing of Food [J]. Transactions of the Chinese Society of Agricultural Engineering, 2007, 23(6): 285-290. (in Chinese)

王标诗, 曾庆孝, 李汴生, 等. 食品超高静压处理中热效应问题的研究进展 [J]. 农业工程学报, 2007, 23(6): 285-290.

Islam M S, Inoue A, Igura N, et al. Inactivation of Bacillus Spores by the Combination of Moderate Heat and Low Hydrostatic Pressure in Ketchup and Potage [J]. Int J Food Microbiol, 2006, 107(2): 124-130.

Zeng Q M, Xie H M, Pan J, et al. Effect of Ultra-High Pressure Processing (UHPP) on the Microstructure of Bacillus subtilis [J]. Chinese Journal of High Pressure Physics, 2006, 20(1): 83-87. (in Chinese)

曾庆梅, 谢慧明, 潘见, 等. 超高压处理对枯草芽孢杆菌超微结构的影响 [J]. 高压物理学报, 2006, 20(1): 83-87.

Okazaki T, Kakugawa K, Yoneda T, et al. Inactivation Behavior of Heat-Resistant Bacterial Spores by Thermal Treatments Combined with High Hydrostatic Pressure [J]. Food Sci Tech Res, 2000, 6(3): 204-207.

Ananta E, Heinz V, Schlter O, et al. Kinetic Studies on High-Pressure Inactivation of Bacillus stearothermophilus Spores Suspended in Food Matrices [J]. Innovat Food Sci Emerg Tech, 2001, 2(4): 261-272.

Lucas R, Grande M J, Abriouel H, et al. Application of the Broad-Spectrum Bacteriocin Enterocin AS-48 to Inhibit Bacillus coagulans in Canned Fruit and Vegetable Foods [J]. Food Chem Toxicol, 2006, 44(10): 1774-1781.

Mallidis C G, Frantzeskakis P, Balatsouras G, et al. Thermal Treatment of Aseptically Processed Tomato Paste [J]. Int J Food Sci Tech, 1990, 25(4): 442-448.

Iciek J, Papiewska A, Molska M. Inactivation of Bacillus stearothermophilus Spores during Thermal Processing [J]. J Food Eng, 2006, 77(3): 404-410.

Liu C M, Zhong Y J, Liu W, et al. Effect of Instantaneous High Pressure on Bacillus stearothemophilus Reductions [J]. Food Science, 2006, 27(9): 55-58. (in Chinese)

刘成梅, 钟业俊, 刘伟, 等. 瞬间高压对嗜热脂肪芽孢杆菌的杀灭效果 [J]. 食品科学, 2006, 27(9): 55-58.

Wang B S, Li B S, Zeng Q X, et al. Inactivation Kinetics of Bacillus stearothermophilus Spore by Pressure-Thermal Processing [J]. Science and Technology of Food Industry, 2009, 30(5): 112-115. (in Chinese)

王标诗, 李汴生, 曾庆孝, 等. 热压协同对嗜热脂肪芽孢杆菌芽孢灭活动力学的研究 [J]. 食品工业科技, 2009, 30(5): 112-115.

Chen X W, Lou X Z, Wang Y X. Influence of Ultra Pressure Processing on the Nutrition Element of Coprinuscomatus [J]. Science and Technology of Food Industry, 2008, 29(7): 228-229. (in Chinese)

陈锡威, 楼雄珍, 王允祥. 超高压对鸡腿菇营养成分的影响 [J]. 食品工业科技, 2008, 29(7): 228-229.

Cole M B, Davies K W, Munro G, et al. A Vitalistic Model to Describe the Thermal Inactivation of Listeria monocytogenes [J]. J Ind Microbiol, 1993, 12(3-5): 232-237.

Chen H, Hoover D G. Use of Weibull Model to Describe and Predict Pressure Inactivation of Listeria monocytogenes Scott A in Whole Milk [J]. Innovat Food Sci Emerg Tech, 2004, 5(3): 269-276.

Ahn J, Balasubramaniam V M, Yousef A E. Inactivation Kinetics of Selected Aerobic and Anaerobic Bacterial Spores by Pressure-Assisted Thermal Processing [J]. Int J Food Microbiol, 2007, 113(3): 321-329.

Black E P, Koziol-Dube K, Guan D S, et al. Factors Influencing Germination of Bacillus subtilis Spores via Activation of Nutrient Receptors by High Pressure [J]. Appl Environ Microb, 2005, 71(10): 5879-5887.

Huang J, Li B S, Wang B S. Study on Effect of High-Hydrostatic Pressure on the Inner-Change of Bacillus Spores [J]. Science and Technology of Food Industry, 2007, 28(9): 219-221. (in Chinese)

黄娟, 李汴生, 王标诗. 超高静压对细菌芽孢内部变化影响的研究 [J]. 食品工业科技, 2007, 28(9): 219-221.

Rajan S, Pandrangi S, Balasubramaniam V M, et al. Inactivation of Bacillus stearothermophilus Spores in Egg Patties by Pressure-Assisted Thermal Processing [J]. LWT-Food Sci Technol, 2006, 39(8): 844-851.

Rajan S, Ahn J, Balasubramaniam V M, et al. Combined Pressure-Thermal Inactivation Kinetics of Bacillus amyloliquefaciens Spores in Egg Patty Mince [J]. J Food Prot, 2006, 69(4): 853-860.

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