响应曲面法优化超高压杀灭金黄色葡萄球菌条件的研究

高瑀珑; 王允祥; 江汉湖

doi:10.11858/gywlxb.2004.03.013

响应曲面法优化超高压杀灭金黄色葡萄球菌条件的研究

doi: 10.11858/gywlxb.2004.03.013

南京农业大学食品科技学院，江苏南京 210095

详细信息

通讯作者:
江汉湖

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出版历程
- 收稿日期: 2003-11-17
- 修回日期: 2004-02-09
- 发布日期: 2004-09-05

Optimization of Conditions to Sterilize Staphyloccus Aureus by Ultra-High Hydrostatic Pressure Using Response Surface Methodology

College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China

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Corresponding author: JIANG Han-Hu

摘要

摘要: 因子试验研究结果表明，显著影响超高压杀灭金黄色葡萄球菌效果的外界因子是温度、压力和保压时间。在此基础上，采用响应曲面法（Response Surface Methodology，RSM）建立了超高压杀灭金黄色葡萄球菌的二次多项数学模型，验证了模型的有效性，并探讨了上述3个因子的交互作用及其最佳水平范围，优化出杀灭6个数量级金黄色葡萄球菌ATCC6538的外界条件为：温度34.35 ℃，压力329.84 MPa，时间15.53 min。
- 超高压杀菌 /
- 金黄色葡萄球菌 /
- 响应面法 /
- 优化
Abstract: The results of factorial experiment showed that the significant external factors affecting UHHP sterilization were pressure, temperature and pressure holding time. Based on the results, response surface methodology (RSM) was employed and a second order quadratic equation for UHHP sterilization was built in the present work. The applicability of the model equation for predicting the optimum response values was verified effectively by the validation data. By analyzing the response surface plots and their corresponding contour plots as well as solving the quadratic equation, the optimum process parameters for sterilization Staphyloccus aureus of six log cycles were obtained as: temperature 34.35 ℃, pressure 329.84 MPa, and pressure holding time15.53 min.
- ultra-high hydrostatic pressure (UHHP) sterilization /
- Staphyloccus aureus /
- response surface methodology (RSM) /
- optimization

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参考文献(14)

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