动态高压微射流提取对红薯叶黄酮抗氧化性的影响

涂宗财; 张露; 王辉; 叶云花; 李志; 黄小琴

doi:10.11858/gywlxb.2013.03.018

动态高压微射流提取对红薯叶黄酮抗氧化性的影响

doi: 10.11858/gywlxb.2013.03.018

涂宗财^1,2, ,,
张露¹,
王辉¹,
叶云花²,
李志¹,
黄小琴¹

1.
南昌大学食品科学与技术国家重点实验室，江西南昌 330047；
2.
江西师范大学，江西南昌 330022

详细信息

通讯作者:
涂宗财 E-mail:tuzc_mail@yahoo.com.cn

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出版历程
- 收稿日期: 2011-10-18
- 修回日期: 2012-01-21
- 发布日期: 2013-06-15

Effects of Dynamic High Pressure Microfluidization (DHPM) Extraction on the Antioxidation of Flavonoids from Sweet Potato Leaves and Mechanism Studying

1.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2.
Jiangxi Normal University, Nanchang 330022, China

摘要

摘要: 采用动态高压微射流（DHPM）辅助技术提取红薯叶黄酮，与传统乙醇提取法比较，研究DHPM对红薯叶黄酮抗氧化活性的影响，并通过高效液相色谱-质谱联用技术（HLPC-MS）技术对其机理进行了初步研究。实验结果显示，DHPM可以促进红薯叶细胞壁的破碎和展开，提高总黄酮的提取率，当处理压力为100 MPa时，总黄酮得率最高（5.440%0.006%），与传统浸提相比，得率提高了21.7%。HLPC-MS分析表明，DHPM可以提高提取黄酮的纯度和低极性分子的浓度，对于同一质量浓度的总黄酮样液，80、100 MPa压力下提取的黄酮样液的抗氧化活性较低，120 MPa压力处理所得样品（SF120）抗氧化活性最强，清除DPPH和OH的IC50值分别为15.99 mg/L和0.093 g/L。SF0、SF80和SF100中的主要黄酮类化合物为槲皮素、4',7-二甲氧基山奈酚、杨梅黄酮、商陆黄素和鼠李柠檬素，SF120的主要黄酮类化合物为槲皮素、杨梅黄酮和商陆黄素。
- 红薯叶 /
- 黄酮 /
- 抗氧化性 /
- 动态超高压微射流 /
- 高效液相色谱-质谱联用技术
Abstract: Effects of dynamic high pressure micro-fluidization(DHPM) in the extraction processing on the antioxidative activities of flavonoid from sweet potato leaves by DHPM in contrast to the traditional ethanol extraction were performed to explore the possible mechanism in combination with the high performance liquid chromatography combined with mass spectrum (HPLC-MS).The results showed that pretreatment by DHPM could promote cell rupture and unfolding of cell membrane, which caused the increase in the extraction rate of total flavonoid. The best result was achieved at 100 MPa with the highest yield of 5.440%0.006%, which was higher by 21.7%, relative to the enthanol extraction. HLPC-MS analysis revealed that pretreatment by DHPM could improve the purity and content of lower polar molecule in extract solvent. 80 MPa and 100 MPa pressurization treatments decreased the antioxidant activity of samples with identical flavonoid content, but had negligible effects on elements and structure of flavonoid. Pretreatment with 120 MPa (SF120 sample) pressurization had the strongest antioxidation with IC50 for DPPH and OH were (15.991.19)mg/L and (0.0930.012)g/L, respectively. The main flavoniods of SF0, SF80 and SF100 are quercetin, 4',7-dimethoxykaempferol, myricetin, ombuin and rhamnocitrin, while, the main flavoniods of SF120 are quercetin, myricetin and ombuin.
- sweet potato leaves /
- flavonoid /
- antioxidative activity /
- dynamic high pressure micro-fluidization (DHPM) /
- high performance liquid chromatography combined with mass spectrum (HPLC-MS)

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