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Volume 27 Issue 4
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Chinese Journal of High Pressure Physics  > 2013  >  27(4): 609-615.
WANG Zhang-Cun, TIAN Wei-Huan, CUI Sheng-Wen, ZHAO Xue-Wei, ZHENG Jian-Qiang, LI Chang-Wen, YUAN Dao-Qiang. Effects of High Hydrostatic Pressure on the Solubility and Molecular Structure of Rice Protein[J]. Chinese Journal of High Pressure Physics, 2013, 27(4): 609-615. doi: 10.11858/gywlxb.2013.04.023
Citation: WANG Zhang-Cun, TIAN Wei-Huan, CUI Sheng-Wen, ZHAO Xue-Wei, ZHENG Jian-Qiang, LI Chang-Wen, YUAN Dao-Qiang. Effects of High Hydrostatic Pressure on the Solubility and Molecular Structure of Rice Protein[J]. Chinese Journal of High Pressure Physics, 2013, 27(4): 609-615. doi: 10.11858/gywlxb.2013.04.023
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Effects of High Hydrostatic Pressure on the Solubility and Molecular Structure of Rice Protein

doi: 10.11858/gywlxb.2013.04.023

  • School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China

  • Received Date: 23 Oct 2011
  • Rev Recd Date: 08 May 2012
  • Issue Publish Date: 15 Aug 2013
    Abstract
  • The effects of high hydrostatic pressure (HHP) treatment at 100-500 MPa for 10-30 min on the solubility and molecular structure characteristics of thermal denatured rice protein dissolved in solvent A (water solution, pH 8.0) and solvent B (containing 6 mol/L urea and 1% mercaptoethanol, pH 8.0) were researched. The results showed that the solubility of rice protein in solvent B was notably improved after HHP treatment, especially at 100 MPa for 20 min, which was from 6.89% at 0.1 MPa up to 18.13%, while in solvent A, it was from 4.37% to 9.60% at the same pressure condition. SDS-PAGE analysis showed that the subunit composition of soluble protein treated under HHP in solvent A had few differences from that of untreated. The similar phenomena could be seen in insoluble protein. However in solvent B, the relative contents of the subunits in soluble changed obviously. The observation of SEM (Scanning Electron Microscope) showed that the surface structure and aggregation state of rice protein could be changed by HHP treatment.

     

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