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Chinese Journal of High Pressure Physics  > 2012  >  26(2): 235-240.
CHEN Xiao-Qiang, ZHANG Yin-Jun, ZHANG Shi-Kang, LI Jian-Rong, JIN Jian-Chang, ZHENG Na, YANG  Xiu-Fang. Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii[J]. Chinese Journal of High Pressure Physics, 2012, 26(2): 235-240. doi: 10.11858/gywlxb.2012.02.018
Citation: CHEN Xiao-Qiang, ZHANG Yin-Jun, ZHANG Shi-Kang, LI Jian-Rong, JIN Jian-Chang, ZHENG Na, YANG  Xiu-Fang. Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii[J]. Chinese Journal of High Pressure Physics, 2012, 26(2): 235-240. doi: 10.11858/gywlxb.2012.02.018
shu

Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii

doi: 10.11858/gywlxb.2012.02.018
  • 1.

    Hangzhou Tea Research Institute, China COOP, Hangzhou 310016, China;

  • 2.

    College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China;

  • 3.

    College of Biological and Envrionmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China;

  • 4.

    College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China;

  • 5.

    State Key Laboratory of Chemical Engineering, Zhejiang University, Hangzhou 310027, China;

  • 6.

    Bohai University, Jinzhou 121013, China

  • Received Date: 17 Nov 2010
  • Rev Recd Date: 17 Feb 2011
  • Issue Publish Date: 15 Apr 2012
    Abstract
  • The activity of polyphenol oxidase (PPO) produced from Trametes Trogii was determined through using tea polyphenols as substrate. The pH value and the substrate concentration of strongest activity of PPO are 5.0 and 4 mg/mL, respectively. The activity of PPO processed by ultrahigh pressure of 100, 300 and 500 MPa for 10 min accounted for 93%, 87% and 86% of that of untreated one. The CD spectra of PPO processed by 100 and 300 MPa pressure respectively displayed folding shape and tillering shape of Cotton effect peak located in 190－210 nm, while the retention time of the first peak fraction(i.e., the maximum molecular weight fraction) of the two processed PPO on HPGPC profiles were advanced to 1.514 and 2.296 min, respectively from 6.678 min. Compared with control, CD spectrum and HPGPC of PPO treaded by 500 MPa showed more significant difference with only one positive Cotton effect peaks on CD spectra and reduced peak area of main components on HPGPC profiles (ie, the response value of 280 nm decreased). The disparity of CD and HPGPC of PPO treated by various UHPP showed the corresponding difference in the degree.

     

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