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Chinese Journal of High Pressure Physics  > 2005  >  19(2): 179-183 .
HUANG Li, SUN Yuan-Ming, PAN Ke, CHEN Bai-Nuan, LIANG Di-Wen, CHEN Guo-Lian, YU Hong-Ying. Influence of Ultra High Pressure on Peroxidase Pectin Methyl Esterase and Soluble Protein in Litchi Fruit[J]. Chinese Journal of High Pressure Physics, 2005, 19(2): 179-183 . doi: 10.11858/gywlxb.2005.02.014
Citation: HUANG Li, SUN Yuan-Ming, PAN Ke, CHEN Bai-Nuan, LIANG Di-Wen, CHEN Guo-Lian, YU Hong-Ying. Influence of Ultra High Pressure on Peroxidase Pectin Methyl Esterase and Soluble Protein in Litchi Fruit[J]. Chinese Journal of High Pressure Physics, 2005, 19(2): 179-183 . doi: 10.11858/gywlxb.2005.02.014
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Influence of Ultra High Pressure on Peroxidase Pectin Methyl Esterase and Soluble Protein in Litchi Fruit

doi: 10.11858/gywlxb.2005.02.014
  • 1.

    College of Food Science, South China Agricultural University, Guangzhou 510642, China;

  • 2.

    College of Industrial Equipment and Control Engineering, South China University of Technology, Guangzhou 510641, China;

  • 3.

    Guangdong Agriculture, Industry and Business Polytechnique College, Guangzhou 510507, China

More Information
  • Corresponding author: SUN Yuan-Ming
  • Received Date: 02 Feb 2004
  • Rev Recd Date: 08 Jun 2004
  • Issue Publish Date: 05 Jun 2005
    Abstract
  • In order to investigate the effects of ultra high pressure (UHP) on the content of soluble protein, peroxidase (POD) and pectinmethylesterase (PME) in litchi fruit, the pulp of litchi (Litchi chinensis Sonn cv. Huaizhi) was subjected to UHP treatment (100~400 MPa for 30 min at 10 ℃). The activities of POD and PME were detected by spectrophotometry, the isoenzymes of POD and PME were detected by native-PAGE and active staining. The content of soluble protein was detected by SDS-PAGE. Results showed that the POD activity raised and the new isoenzyme appeared when a treatment of 100~200 MPa was applied, the treatment of pressure 300~400 MPa made the POD activity decrease and the new isoenzyme disappear. The PME activity ascended and the new isoenzyme of PME appeared at 100 MPa; when the treatment of 200~400 MPa was applied the PME activity lowered and the new isoenzyme disappeared. The content of soluble protein had a little increase at 100 MPa, but continue to declined at 200~400 MPa.

     

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