Preparation and Characterization of Glucose Liposome by Supercritical Fluid Technology

Liposomes have proven to be promising carriers for their special structure and characterization, and can be widely used in pharmacy and cosmetics.Supercritical reverse phase evaporation (SRPE) is a new technique for preparing liposomes with supercritical fluid instead of organic solvent.The process has characteristic of simple and high coating ratio for water-soluble substances.The technique was used to prepare liposomes with water soluble glucose, and the effects of the process parameters on the particle size and the coating ratio of liposomes were investigated in detail.The liposomes with an average volume diameter of 290-330 nm were successfully prepared by this technique and the coating ratio for water-soluble glucose was as high as 41.3%.It is confirmed that the temperature, pressure and equilibrium time have great effects on the particle size and the coating ratio.As the pressure increased from 10 to 30 MPa, the particle size first decreased remarkably and then kept approximately constant, while the coating ratio gradually increased to 25.7%-27.5% and then gradually decreased; as the temperature increased from 35 to 65 ℃, the particle size decreased obviously to its minimum value and then gradually increased, while the coating ratio increased all along, even to 41.3%.With the equilibrium time increasing from 15 to 45 min, the particle size kept relatively stable, while the coating ratio increased remarkably; when the equilibrium time exceeded 45 min, the particle size increased remarkably, while the coating ratio decreased remarkably.