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Chinese Journal of High Pressure Physics  > 2012  >  26(5): 595-600.
GONG Wu-Xia, LI Shu-Yan, JIANG Jun, CHEN Fang, ZHANG Yan, HU Xiao-Song, WU Ji-Hong. Effect of Pressure Combined with Metal Ions on Polyphenol Oxidase (PPO) Activity from Raspberry[J]. Chinese Journal of High Pressure Physics, 2012, 26(5): 595-600. doi: 10.11858/gywlxb.2012.05.018
Citation: GONG Wu-Xia, LI Shu-Yan, JIANG Jun, CHEN Fang, ZHANG Yan, HU Xiao-Song, WU Ji-Hong. Effect of Pressure Combined with Metal Ions on Polyphenol Oxidase (PPO) Activity from Raspberry[J]. Chinese Journal of High Pressure Physics, 2012, 26(5): 595-600. doi: 10.11858/gywlxb.2012.05.018
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Effect of Pressure Combined with Metal Ions on Polyphenol Oxidase (PPO) Activity from Raspberry

doi: 10.11858/gywlxb.2012.05.018

  • College of Food Science and Nutritional Engineering, China Agricultural University; Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture; Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, Beijing 100083, China

  • Received Date: 17 Nov 2010
  • Rev Recd Date: 28 Feb 2011
  • Publish Date: 15 Oct 2012
    Abstract
  • The activity of polyphenol oxidase from red raspberry treated at 600 MPa for 45 min is even up to 40%, indicating strong pressure resistance. In order to further inactivate polyphenol oxidase from red raspberry, the combination effect of high hydrostatic pressure and metal ions on polyphenol oxidase activity was investigated. The change of polyphenol oxidase activity treated at 400 and 600 MPa after adding metal ions was revealed. The activity of raspberry polyphenol oxidase gradually decreases with the pressure increasing and/or the holding time extending with/without adding metal ions. Fe2+, Mn2+ (both inhibited polyphenol oxidase) and Cu2+ (activated polyphenol oxidase) in combination with pressure have synergistic effects, Ca2+ (inhibited polyphenol oxidase) combined with pressure has antagonistic effect, and Zn2+ (not change polyphenol oxidase activity) has no synergistic/antagonistic effect.

     

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