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Chinese Journal of High Pressure Physics  > 2010  >  24(2): 129-135 .
LIU Wei, ZHONG Ye-Jun, LIU Cheng-Mei, XIE Ming-Yong, GUAN Bin, YIN Man, WANG Qian, CHEN Ting-Ting. The Effect of Dynamic High Pressure Microfluidization on the Activity of Papain[J]. Chinese Journal of High Pressure Physics, 2010, 24(2): 129-135 . doi: 10.11858/gywlxb.2010.02.009
Citation: LIU Wei, ZHONG Ye-Jun, LIU Cheng-Mei, XIE Ming-Yong, GUAN Bin, YIN Man, WANG Qian, CHEN Ting-Ting. The Effect of Dynamic High Pressure Microfluidization on the Activity of Papain[J]. Chinese Journal of High Pressure Physics, 2010, 24(2): 129-135 . doi: 10.11858/gywlxb.2010.02.009
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The Effect of Dynamic High Pressure Microfluidization on the Activity of Papain

doi: 10.11858/gywlxb.2010.02.009

  • State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

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  • Corresponding author: LIU Cheng-Mei
  • Received Date: 23 Feb 2009
  • Rev Recd Date: 07 Apr 2009
  • Publish Date: 15 Apr 2010
    Abstract
  • The effect of dynamic high pressure microfluidization (DHPM) on the activity of papain had been investigated, and the molecular conformation changes of papain had been detected by fluorescence spectra. The results showed that after DHPM (120～180 MPa) treatment, the enzyme activity decreased in different degrees. The papain activity reduced to 90.04% after 180 MPa treatment. With the increasing of treatment pressure, the fluorescence emission peaks of papain, Tyr and Trp residues gradually red shifted from 334, 285 and 277.5 nm for untreated sample to 335.5, 285.5 and 278.5 nm for 140 MPa treated sample, and then returned to 334, 285 and 278 nm for 180 MPa treated sample, respectively. After 24 h placing at 0～4 ℃, papain activity further decreased and the fluorescence intensity of papain and Tyr residue showed oscillate (down, up and down), which indicated that DHPM treatment (120～180 MPa) changed the molecular conformation of papain visibly with low stability of the new formed conformation.

     

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