Effects of Dynamic Instantaneous High Pressure Treatment on the enzymolysis rate of Dietary Fiber

The enzymolysis rates of cellulase on dietary fiber under dynamic Instantaneous High Pressure (IHP) were studied in the paper. Utilizing the Microfluidizer machinery as the carrier of Instantaneous High Pressure treatment to deal with dietary fiber from bean dregs, we got dietary fiber of different granularities and densities under different pressures and different times with the same pressure. The corresponding enzymolysis rates of cellulase on dietary fiber were different. The hydrolysates of cellulase on dietary fiber were some reducing sugars, such as cellobiose and glucose. The enzymolysis rates could be judged by measuring the contents of the reducing sugars in the dietary fiber of different granularities and densities. The results indicated that with the increase of the pressure in the range of 40～90 MPa, the granularities of the materials reduced greatly, the densities of the materials and the enzymolysis rates increased. And the granularity of the materials was in minimum at 202.4 nm; when the pressure went on increasing, the granularities of the materials tended to increase because of the occurrence of expanding in the system, but the densities started to decreased and the enzymolysis rates kept increasing. The density of the materials was in minimum at 1.027 g/mL, the contents of the reducing sugars after enzyme hydrolysis was maximal at this time. When the pressure higher than 140 MPa, the granularities and density of the materials increased, the enzymolysis rates decreased. It could be due to the reuniting of particles in the system. When the materials were treated separately with instantaneous high pressure between 90 MPa and 140 MPa for several times, the granularities of the materials increased as the increase of dealing times, the density decreased at the start and then increased, and the enzymolysis rates decreased.