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Chinese Journal of High Pressure Physics  > 2007  >  21(1): 89-94 .
HUANG Li, SUN Yuan-Ming, CHEN Bai-Nuan, HUANG Wei, ZHAN Guo-Lian. Influence of Combined Treatment of Ultra High Pressure and Enzyme Inhibitors on Peroxidase and Pectin Methyl Esterase in Litchi Pulp[J]. Chinese Journal of High Pressure Physics, 2007, 21(1): 89-94 . doi: 10.11858/gywlxb.2007.01.015
Citation: HUANG Li, SUN Yuan-Ming, CHEN Bai-Nuan, HUANG Wei, ZHAN Guo-Lian. Influence of Combined Treatment of Ultra High Pressure and Enzyme Inhibitors on Peroxidase and Pectin Methyl Esterase in Litchi Pulp[J]. Chinese Journal of High Pressure Physics, 2007, 21(1): 89-94 . doi: 10.11858/gywlxb.2007.01.015
shu

Influence of Combined Treatment of Ultra High Pressure and Enzyme Inhibitors on Peroxidase and Pectin Methyl Esterase in Litchi Pulp

doi: 10.11858/gywlxb.2007.01.015
  • 1.

    College of Food Science, South China Agricultural University, Guangzhou 510642, China;

  • 2.

    Guangdong Agriculture, Industry and Business Polytechnic College, Guangzhou 510507, China;

  • 3.

    College of Industrial Equipment and Control Engineering, South China University of Technology, Guangzhou 510641, China

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  • Corresponding author: SUN Yuan-Ming
  • Received Date: 15 Dec 2005
  • Rev Recd Date: 17 Apr 2006
  • Publish Date: 05 Mar 2007
    Abstract
  • In order to investigate the effects of combined treatment of ultra high pressure (UHP) and enzyme inhibitors on the activities of peroxidase (POD) and pectin methyl esterase (PME) in litchi pulp, two kinds of enzyme inhibitor prescriptions were designed including group A (citric acid 5 g/L+L-ascorbic acid 2.5 g/L+calcium chloride 5 g/L) and group B (citric acid 10 g/L+L-ascorbic acid 5 g/L +calcium chloride 10 g/L). After being immersed in enzyme inhibitor solutions for 10 min, the pulp of litchi (Litchi chinensis Sonn cv. Huaizhi) was subjected to UHP treatment (100~400 MPa for 30 min at 10 ℃). The activities of POD and PME were detected by spectrophotometry. Results showed that separate treatment of group A or group B could lead to obvious passivation of POD and prominent activation of PME. Combined treatment of UHP and group A could not make the activities of POD and PME decrease. The influence of combined treatment of UHP and group B on POD and PME had relevance to pressure value, the POD activity lowered when the combined treatment of 100~300 MPa and group B was applied, and the PME activity raised obviously when the combined treatment of 200~400 MPa and group B was applied. So, combined treatment of UHP and enzyme inhibitors had certain synergistic effect for POD inactivation, and the synergistic inhibition effect would be more obvious with higher concentration of enzyme inhibitors; but combined treatment of UHP and enzyme inhibitors showed certain rivalry for PME inactivation.

     

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    • References(31)
  • Phunchaisri C, Apicharsrangkoon A. Effects of Ultra-High Pressure on Biochemical and Physical Modification of Lychee(Litchi chinensis Sonn) [J]. J Food Chemistry, 2005, 93: 57-64.
    Krebbers B, Master A M, Koets M, et al. Quality and Storage-Stability of High-Pressure Preserved Green Beans [J]. J Food Eng, 2002, 54: 27-33.
    Smelt J P P M. Recent Advances in the Microbiology of High Pressure Processing [J]. J Food Sci Tech, 1998, 9: 152-158.
    Estrada-Giron Y, Swanson B G, Barbosa-Canovas G V. Advances in the Use of High Hydrostatic Pressure for Processing Cereal Grains and Legumes [J]. J Food Sci Tech, 2005, 16: 194-203.
    Sancho F, Lambert Y, Demazeau G, et al. Effect of Ultra-High Hydrostatic Pressure on Hydrosoluble Vitamins [J]. J Food Eng, 1999, 39: 247-253.
    Yuste J, Capellas M, Pla R, et al. High Pressure Processing for Food Safety and Preservation: A Review [J]. J Rapid Methods and Automation in Microbiology, 2001, 9(1): 1-10.
    Sumitani H, Suekane S, Nakatani A, et al. Changes in Composition of Volatile Compounds in High Pressure Treated Peach [J]. J Agric Food Chem, 1994, 42: 785-790.
    Kimura K, Ida M, Yosida Y, et al. Comparison of Keeping Quality between Pressure-Processed Jam and Heat-Processed Jam: Changes in Flavor Components, Hue and Nutrients during Storage [J]. J Biosci Biotech Biochem, 1994, 58(8): 1386-1391.
    Rastogl N K, Niranjan K. Enhanced Mass Transfer during Osmotic Dehydration of High Pressure Treated Pineapple [J]. J Food Sci, 1998, 63(3): 508-511.
    Yen G C, Lin H T. Changes in Volatile Flavor Components of Guava Juice with High-Pressure Treatment and Heat Processing and during Storage [J]. J Agric Food Chem, 1999, 47: 2082-2087.
    Tangwongehai R, Ledward D A, Ames J M. Effect of High-Pressure Treatment on the Texture of Cherry Tomato [J]. J Agric Food Chem, 2000, 48: 1434-1441.
    MacDonald L, Schaschke C J. Combined Effect of High Pressure, Temperature and Holding Time on Polyphenoloxidase and Peroxidase Activity in Banana(Musa acuminata) [J]. J Sci Food Agric, 2000, 80: 719-724.
    Kim Y S, Park S J, Cho Y H, et al. Effects of Combined Treatment of High Hydrostatic Pressure and Mild Heat on the Quality of Carrot Juice [J]. J Food Eng Phys Proper, 2001, 66(9): 1355-1360.
    Polydera A C, Stoforos N G, Taoukis P S. Comparative Shelf Life Study and Vitamin C Loss Kinetics in Pasteurised and High Pressure Processed Reconstituted Orange Juice [J]. J Food Eng, 2003, 60: 21-29.
    Zeng Q M, Pan J, Xie H M, et al. Influence of Ultra-High-Pressure (UHP) on Micro-Organisms in Watermelon Juice [J]. Chinese Journal of High Pressure Physics, 2004, 18(1): 70-74. (in Chinese)
    曾庆梅, 潘见, 谢慧明, 等. 西瓜汁的超高压杀菌效果研究 [J]. 高压物理学报, 2004, 18(1): 70-74.
    Huang L, Sun Y M, Pan K, et al. Influence of Ultra High Pressure on Peroxidase, Pectinmethylesterase and Soluble Protein in Litchi Fruit [J]. Chinese Journal of High Pressure Physics, 2005, 19(2): 179-183. (in Chinese)
    黄丽, 孙远明, 潘科, 等. 超高压处理对荔枝果肉中可溶性蛋白和两种酶的影响 [J]. 高压物理学报, 2005, 19(2): 179-183.
    Prestamo G, Arabas J, Broczek M F, et al. Reaction of B. cereus Bacteria and Peroxidase Enzyme under Pressure 400 MPa [J]. J Agric Food Chem, 2001, 49: 2830-2834.
    Alonso J, Rodriguez T, Canet W. Purification and Characterization of Two Pectinmethylesterase from Persimmon(Diospyros kaki) [J]. J Sci Food Agric, 1997, 75: 352-358.
    Pan K, Sun Y M, Huang L, et al. Effects of Some Factors on Peroxidase and Lipoxygenase Activity in Longan [J]. Food and Fermentation Industries, 2002, 28(6): 13-17. (in Chinese)
    潘 科, 孙远明, 黄丽, 等. 几种物质对龙眼果肉中过氧化物酶和脂肪氧合酶的影响 [J]. 食品与发酵业, 2002, 28(6): 13-17.
    Cheng J J, Ren Y H, Yang Y L. Recent Progress in Inhibition of Enzymatic Browning of Fruit and Vegetable [J]. Journal of Northeast Agricultural University, 2000, 31(4): 406-410. (in Chinese)
    程建军, 任运宏, 扬咏丽, 等. 果蔬酶褐变控制的研究进展 [J]. 东北农业大学学报, 2000, 31(4): 406-410.
    Prestamo G, Manzano P. Peroxidases of Selected Fruits and Fegetables and the Possible Use of Ascorbic Acid as an Antioxidant [J]. HortScience, 1993, 28: 48-50.
    Alonso J, Rodriguez T, Canet W. Effect of Calcium Pretreatments on the Texture of Frozen Cherries. Role of Pectinesterase in the Changes in the Pectic Materials [J]. J Agric Food Chem, 1995, 43: 1011-1016.
    Alonso J, Canet W, Rodriguez T. Thermal and Calcium Pretreatment Affects Texture, Pectinesterase and Pectic Substances of Frozen Sweet Cherries [J]. J Food Sci, 1997, 62: 511-515.
    Weemaes C A, Ludikhuyze L R, Broeck I E, et al. Influence of pH, Benzoic Acid, Glutathione, EDTA, 4-Hexylresorcinol and Sodium Chloride on the Pressure Inactivation Kinetics of Mushroom Polyphenol Oxidase [J]. J Agric Food Chem, 1999, 47: 3526-3530.
    Prestamo G, Arroyo G. Protective Effect of Ascorbic Acid against the Browning Developed in Apple Fruit with High Hydrostatic Pressure [J]. J Agric Food Chem, 1997, 45: 3541-3545.
    Broeck I E, Ludikhuyze L R, Ven Loey A M, et al. Thermal and Combined Pressure-Temperature Inactivation of Orange Pectinesterase: Influence of pH and Additives [J]. J Agric Food Chem, 1999, 47: 2950-2958.
    Broeck I E, Ludikhuyze L R, Ven Loey A M, et al. Inactivation of Orange Pectinesterase by Combined Pressure-Temperature: A Kinetic Study [J]. J Agric Food Chem, 2000, 48: 1960-1970.
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