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Chinese Journal of High Pressure Physics  > 2006  >  20(1): 83-87 .
ZENG Qing-Mei, XIE Hui-Ming, PAN Jian, YANG Yi, XU Hui-Qun, HUANG Xun-Duan, ZHAO Yang-Nan. Effect of Ultra-High Pressure Processing (UHPP) on the Microstructure of Bacillus subtilis[J]. Chinese Journal of High Pressure Physics, 2006, 20(1): 83-87 . doi: 10.11858/gywlxb.2006.01.016
Citation: ZENG Qing-Mei, XIE Hui-Ming, PAN Jian, YANG Yi, XU Hui-Qun, HUANG Xun-Duan, ZHAO Yang-Nan. Effect of Ultra-High Pressure Processing (UHPP) on the Microstructure of Bacillus subtilis[J]. Chinese Journal of High Pressure Physics, 2006, 20(1): 83-87 . doi: 10.11858/gywlxb.2006.01.016
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Effect of Ultra-High Pressure Processing (UHPP) on the Microstructure of Bacillus subtilis

doi: 10.11858/gywlxb.2006.01.016
  • 1.

    Key Laboratory of Bio-Process (Hefei University of Technology), Ministry of Education, Hefei 230009, China;

  • 2.

    Anhui Province Import and Export Commodity Inspection and Quarantine Bureau, Hefei 230006, China

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  • Corresponding author: ZENG Qing-Mei
  • Received Date: 09 Oct 2004
  • Rev Recd Date: 28 Nov 2005
  • Publish Date: 05 Mar 2006
    Abstract
  • The effect of ultra-high pressure processing on the microstructure of Bacillus subtilis AS 1.140 was investigated, and the inactivation mechanism of its vegetative form and spore was studied. The change of B. subtilis microstructures were analyzed and compared in terms of the oberation with a Transmission Electron Microscope (TEM) after UHPP (500 MPa, 60 ℃, 20 min). Results showed that the vegetative cell wall of B.subtilis was shrinked, breached and leaked out cytoplasm, the layer structure of cytoplasm disappeared, and a large electron transmission area appeared in the cell after compression. The outer coat of its spores was also breached, the layer structure of spores' inclusion disordered, the inclusion leaked out from the cell, and a partial electron transmission area appeared after compression.

     

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