Optimization of Conditions to Sterilize Staphyloccus Aureus by Ultra-High Hydrostatic Pressure Using Response Surface Methodology

The results of factorial experiment showed that the significant external factors affecting UHHP sterilization were pressure, temperature and pressure holding time. Based on the results, response surface methodology (RSM) was employed and a second order quadratic equation for UHHP sterilization was built in the present work. The applicability of the model equation for predicting the optimum response values was verified effectively by the validation data. By analyzing the response surface plots and their corresponding contour plots as well as solving the quadratic equation, the optimum process parameters for sterilization Staphyloccus aureus of six log cycles were obtained as: temperature 34.35 ℃, pressure 329.84 MPa, and pressure holding time15.53 min.