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Volume 17 Issue 4
Apr 2015
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Chinese Journal of High Pressure Physics  > 2003  >  17(4): 283-289 .
BAI Cheng-Ke, LI Gui-Shuang, DUAN Jun, PENG Chang-Lian, DUAN Zhong-Gang, WENG KeNan, XU Shi-Ping. Effect of High Hydrostatic Pressure on Seeds Germination and Seedling Isoenzyme in Rice (Oryza sativa L.)[J]. Chinese Journal of High Pressure Physics, 2003, 17(4): 283-289 . doi: 10.11858/gywlxb.2003.04.007
Citation: BAI Cheng-Ke, LI Gui-Shuang, DUAN Jun, PENG Chang-Lian, DUAN Zhong-Gang, WENG KeNan, XU Shi-Ping. Effect of High Hydrostatic Pressure on Seeds Germination and Seedling Isoenzyme in Rice (Oryza sativa L.)[J]. Chinese Journal of High Pressure Physics, 2003, 17(4): 283-289 . doi: 10.11858/gywlxb.2003.04.007
shu

Effect of High Hydrostatic Pressure on Seeds Germination and Seedling Isoenzyme in Rice (Oryza sativa L.)

doi: 10.11858/gywlxb.2003.04.007
  • 1.

    South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650, China;

  • 2.

    Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China

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  • Corresponding author: BAI Cheng-Ke
  • Received Date: 05 Mar 2003
  • Rev Recd Date: 09 Jun 2003
  • Publish Date: 05 Dec 2003
    Abstract
  • Three indica rice cultivars seeds, Yuexiangzhan were treated by different high hydrostatic pressure or were kept for different time. As comparison to control, both germination rate and seedling rate decreased considerably with pressure or treated time increasing. Plant growth was inhibited in treated materials during the early growth stage of seedling, but the plant height of treated cultivars could exceed that of control after sowing 30 d. The expression of some isoenzymes, esterase (EST), peroxidase (POX), superoxide dismutase (SOD) and malic enzyme (ME), was studied by polyacrylamide gel electrophoresis (PAGE), the results indicated that the expression of EST was restrained, whereas the other isoenzymes was affected lettle on expression of POX, SOD and ME. Three types of rice (Oryza sativa L.) cultivars seeds, indica cultivars (Nanjing 11 and Yuexiangzhan), japonica cultivars (Qiuguang and Taizhong 65) and intermediate type between indica cultivars and japonoca cultivars (Bulu Gendjah and Bulu Rusa), were treated by 75 MPa pressure for 10 h. The germination rate and seedling rate of three types rice all decreased. But the decrease was most significant in two indica cultivars (Nanjing 11 and Yuexiangzhan), whereas was least in two japonica cultivars (Qiuguang and Taizhong 65). The results implied that different types of rice cultivars exhibited different responses to high hydrostatic pressure. Japinica cultivars were higher resistance to high pressure than other types of cultivars. The above results showed that high hydrostatic pressure could affect the seeds germination, seedling growth and expression of EST.

     

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    • References(33)
  • Winter R. High Pressure Effects in Molecular Biophysics [A]. Proceedings of the International School of Physics Enrico Fermi, Course CXLVII on High Pressure Phenomena [C]. Amsterdam: IOS, 2002: 413-453.
    Panick G, Winter R. Pressure-Induced Unfolding/Refolding of Ribonuclease A-a Statics and Kinetik Fourier-Transform Infrared Spectroscopy Study [J]. Biochemistry, 2000, 39: 1862-1869.
    Shantha D S, Doulas M C, Ana J, et al. High Resolution NMR Study of the Pressure-Induced Unfolding of Lysozyme [J]. Biochemistry, 1992, 31: 7773-7778.
    Whelch T J, Farewell A, Neidhandt F C, et al. Stress Response of E Coli to Elevated Hydrostatic Pressure [J]. J Bacteriol, 1993, 175: 7170-7177.
    Arroyo G, Sanz P D, Prestamo G. Effect of High Pressure on the Reduction of Microbial Populations in Vegetables [J]. J Appl Microbiol, 1997, 82(6): 735-742.
    Xu S P, Liao Y P, Weng K N, et al. Pressure Induced Rice Mutation and Effects of High Hydrostatic Pressure on the Growth and Development of Rice [J]. Chinese Journal of High Pressure Physics, 2001, 15(4): 241-247. (in Chinese)
    徐世平, 廖耀平, 翁克难, 等. 水稻压致变异和高压对水稻生长发育的影响 [J]. 高压物理学报, 2001, 15(4): 241-247.
    Xu S P, Liao Y P, Xiao W S, et al. Effects of High Pressure on the Growth and Development of Rice [J]. Chinese Journal of High Pressure Physics, 1999, 13(Suppl): 58-63. (in Chinese)
    徐世平, 廖耀平, 肖万生, 等, 高压对水稻生长发育的影响 [J]. 高压物理学报, 1999, 13(增刊): 58-63.
    Bradford M M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [J]. Anal Biochem, 1976, 72: 248-254.
    Sun X L, Cai H W, Wang X K. A Method for Studing Electrophoretic Variation of Isozymess in Rice with Poly-Acrylamide Gel [J]. Chinese Journal Rice Science, 1996, 10(1): 43-50. (in Chinese)
    孙新立, 才宏伟, 王象坤. 水稻同工酶聚丙烯酰胺凝胶电泳方法探索 [J]. 中国水稻科学, 1996, 10(1): 43-50.
    Pan R C. The Rice Physiology [M]. Beijing: Science Press, 1979: 218-224. (in Chinese)
    潘瑞炽. 水稻生理 [M]. 北京: 科学出版社, 1979: 218-224.
    Jiao D M, Ji B H. Changes of the Photosynthetic Electron Transport and Pthotosynthetic Enzyme Activities of Two Rice Varieties under Photooxidation Condition [J]. Acta Agronomica Sinica, 1996, 22(1): 43-49. (in Chinese)
    焦德茂, 季本华. 光氧化条件下两个水稻品种光合电子传递和光合酶活性的变化 [J]. 作物学报, 1996, 22(1): 43-49.
    Pan R C, Ji B H. Plant Physiology (3rd ed) [M]. Beijing: High Education Press, 1998: 237-240. (in Chinese)
    潘瑞炽, 董愚得. 植物生理学(第3版) [M]. 北京: 高等教育出版社, 1995: 237-240.
    Shen T, Wang J Y. Plant Biochemistry (2nd ed)[M]. Beijing: High Education Press , 1990: 234-236. (in Chinese)
    沈同, 王镜岩. 生物化学. 上册(第2版) [M]. 北京: 高等教育出版社, 1990: 234-236.
    Panick G, Winter R. Pressure-Induced Unfolding/Refolding of Ribonuclease a-a Static and Kinetik Fourier-Transform Infrared Spectroscopy Study [J]. Biochemistry, 2000, 39: 1862-1869.
    Desai G, Panick G, Zein M, et al. Pressure-Jump Studies of the Folding/Unfolding of trp Repressor [J]. J Mol Biol, 1999, 288: 461-475.
    Kayo C, Smorawinaka M, Li L, et al. Comparison of the Gene Expression of Aspartate Bate-D-Smialdehyde Dehydrogenase at Elevated Hydrostatic Pressure in Deep-Sea [J]. Bacteria J Biochem, 1997, 121(4): 717-723.
    Malone A S, Shellhammer T H , Courtney P D. Effects of High Pressure on the Viability, Morphology, Lysis, and Cell Wall Hydrolase Activity of Lactococcus Lactis Subsp Cremoris [J]. Appl Environ Microb, 2002, 68(9): 4357-4363.
    Hu N S, Wan G X. The Technology and Application of Isoenzyme [M]. Changsha: Hunan Technology Press, 1985: 96-99. (in Chinese)
    胡能书, 万贤国. 同工酶技术及其应用 [M]. 长沙: 湖南科学技术出版社, 1985: 96-99.
    Siegal B Z, Galston A W. The Isoperoxidases of Pisum Sativum [J]. Plant Physiol, 1967, 42: 221-226.
    Bai C K, Li G S, Peng C L, et al. Preliminary Study of Rice Mutants Induced by High Hydrostatic Pressure [J]. Journal of Tropical and Subtropical Botany, 2003, 11(2): 132-136. (in Chinese)
    白成科, 李桂双, 彭长连, 等. 高静水压诱导水稻变异的初步研究 [J]. 热带亚热带植物学报, 2003, 11(2): 132-136.
    Bai C K, Li G S, Peng C L, et al. High Hydrostatic Pressure Induced Changes of Chlorophyll Fluorescence Rice Mutant Lines [J]. Chinese Journal Rice Sciene, 2003, 17(3): 228-232. (in Chinese)
    白成科, 李桂双, 彭长连, 等. 高静水压诱变水稻突变株系的叶绿素荧光特性变化 [J]. 中国水稻科学, 2003, 17(3): 228-232.
    Li G S, Bai C K, Duan J. Effect of High Hydrostatic Pressure Treatment on Physiological Characteristics of Rice Plants ( Oryza sativa L. ) [J]. Chinese Journalof High Pressure Physics, 2003, 17(2): 122-128. (in Chinese)
    李桂双, 白成科, 段俊, 等. 静水高压处理对水稻植株生理特性的影响 [J]. 高压物理学报, 2003, 17(2): 122-128.
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