Comparison of Various Physical Fields Treatment on Phosphatides Dispersions
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摘要: 利用动态光散射研究了脉冲电场、超声波、微射流3种物理场处理对磷脂分散体系的颗粒性质及稳定性的影响。结果表明:当施加的处理条件分别为60 kV/cm脉冲电场、500 W超声波及130 MPa微射流时,磷脂的平均粒径由594.4 nm分别减小至259.2、88.7和37.8 nm;静置24 h后,脉冲电场处理样的粒径恢复得较好,而超声波和动态高压微射流处理样基本不恢复。说明维系细胞膜结构与流动性功能的磷脂的粒径随外加物理场能量的输入而逐渐减小,高强脉冲电场处理比较柔和,处理后磷脂粒径的变化表现出较强的可逆性,与脉冲电场对生物细胞膜影响的可逆性相对应。Abstract: The particle and stability properties of phosphatides dispersions affected by pulsed electric field, ultrasound, microfluid were studied using dynamic light scattering techniques.Results indicate that the average diameter of phosphatides particles decrease from 594.4 nm to 259.2, 88.7, 37.8 nm after being treated by 60 kV/cm pulsed electric field, 500 W ultrasound and 130 MPa microfluid, respectively.As for the stability of the treated phosphatides dispersion, it is demonstrated that the ultrasound treated sample is the most stable one, then following microfluid and pulsed electric field treated samples.
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Key words:
- pulsed electric field /
- ultrasound /
- microfluid /
- phosphatides dispersions /
- cell membrane
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表 1 超声波处理下磷脂分散体颗粒的平均粒径dm
Table 1. Effects of ultrasound intensities on mean diameter (dm) of phosphatides
P/(W) dm/(nm) De/(J/mL) t=0 t=24 h 0 594.4±3.2 687.7±22.0 0 200 93.7±1.4 93.2±1.1 570 300 89.8±0.1 90.3±0.9 855 400 87.6±0.2 88.4±1.3 1 140 500 88.7±0.3 88.1±0.3 1 425 表 2 微射流作用下磷脂分散体系颗粒的平均粒径dm
Table 2. Effects of microfluid on mean diameter (dm) of phosphatides
p/(MPa) dm/(nm) De/(J/mL) t=0 t=24 h 0 594.4±3.2 687.7±22.0 0 10 74.7±0.9 74.9±0.6 100 30 60.8±0.4 61.2±0.2 300 45 58.0±0.2 58.0±0.5 450 65 49.3±0.2 49.1±0.1 650 95 44.5±0.2 46.9±0.2 950 130 37.8±0.5 38.4±0.7 1 300 -
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