Preparation of Medium-Chain Fatty Acids (MCFA) Nano-Liposome by Means of High Pressure Microfluidization (HPM)
-
摘要: 以中链脂肪酸(MCFA)为模型材料,比较了高压微射流(HPM)、超声波和微孔过滤3种方法处理后的MCFA脂质体,着重研究了HPM的处理压力和处理次数对脂质体的平均粒度、包封率和稳定性的影响。结果表明:相比超声波和微孔过滤法,HPM处理后的MCFA脂质体的平均粒度最小、包封率最高、稳定性良好;在120 MPa压力条件下处理6次时,MCFA脂质体的平均粒度达到最小,为(73.910.2) nm,包封率为(52.209.57)%;在120 MPa压力条件下处理4次时,包封率和载药量达到最高,分别为(70.6411.25)%和(9.420.83)%,稳定性系数最高,为0.999 03.695 1,平均粒度为(78.921.5) nm。Abstract: Medium-chain fatty acids (MCFA) nano-liposome was prepared and characterized by means of high pressure microfluidization (HPM), sonication and microfiltration, respectively. The average diameter, encapsulated efficiency and stability were investigated after treatment with different pressures and passes number. The results indicated that MCFA liposome prepared by HPM exhibited the smallest average diameter, the largest encapsulated efficiency and the best stability. The smallest average diameter of MCFA liposome could be achieved after a treatment of 6 passes at 120 MPa, with a value of (73.910.2) nm, while the encapsulated efficiency was (52.209.57)%. The largest encapsulated efficiency of (70.6411.25)% and loaded efficiency of (9.420.83)% were obtained when treated 4 passes at 120 MPa, with an average diameter value of (78.921.5) nm. After being treated 4 passes, MCFA liposome exhibited the best stability, with a coefficient value of 0.999 03.695 1.
-
Paul P. Technological Properties of High Pressure Homogenizers: The Effect of Fat Globules, Milk Proteins, and Polysaccharides [J]. Int Dairy J, 1999, 9(3-6): 329-335. Liu W, Liu J H, Xie M Y, et al. Characterization and High-Pressure Microfluidization-Induced Activation of Polyphenoloxidase from Chinese Pear (Pyrus pyrifolia Nakai) [J]. J Agric Food Chem, 2009, 57(12): 5376-5380. Feijoo S C, Hayes W W, Watson C E, et al. Effects of Microfluidizer Technology on Bacillus Licheniformis Spores in Ice Cream Mix [J]. J Dairy Sci, 1997, 80(9): 2184-2187. Masson G. Advanced Techniques for Preparation and Characterization of Small Unilamellar Vesicles [J]. Food Microstruct, 1989, 8: 11-14. Thompson A K, Singh H. Preparation of Liposomes from Milk Fat Globule Membrane Phospholipids Using a Microfluidizer [J]. J Dairy Sci, 2006, 89: 410-419. Thiebaud M, Dumay E, Picart L, et al. High-Pressure Homogenisation of Raw Bovine Milk. Effects on Fat Globule Size Distribution and Microbial Inactivation [J]. Int Diary J, 2003, 13(6): 427-439. Wan J, Liu C M, Lan H J, et al. Effects of Dynamic Instantaneous High Pressure Treatment on the Enzymolysis Rate of Dietary Fiber [J]. Chinese Journal of High Pressure Physics, 2008, 22(4): 439-444. (in Chinese) 万婕, 刘成梅, 蓝海军, 等. 动态瞬时高压作用对膳食纤维酶解速度的影响 [J]. 高压物理学报, 2008, 22(4): 439-444. Liu W, Liu J H, Liu C M, et al. Activation and Conformational Changes of Mushroom Polyphenoloxidase by High Pressure Microfluidization Treatment [J]. Innovat Food Sci Emerg Tech, 2009, 10(2): 142-147. Lasic D D, Papahadjopoulos D. Liposomes Revisited [J]. Science, 1995, 267(5202): 1275-1276. Torchilin V P. Recent Advances with Liposomes as Pharmaceutical Carriers [J]. Nat Rev Drug Discov, 2005, 4(2): 145-160. Schmidinger M, Wenzel C, Locker G J, et al. Pilot Study with Pegylated Liposomal Doxorubicin for Advanced or Unresectable Hepatocellular Carcinoma [J]. Br J Cancer, 2001, 85(12): 1850-1852. Wollina U, Dummer R, Brockmeyer N H, et al. Multicenter Study of Pegylated Liposomal Doxorubicin in Patients with Cutaneous T-Cell Lymphoma [J]. Cancer, 2003, 98(5): 993-1001. Liu N, Park H J. Chitosan-Coated Nanoliposome as Vitamin E Carrier [J]. J Microencapsul, 2009, 26(3): 235-242. Bangham A D, Standish M M, Watkins J C. Diffusion of Univalent Ions across the Lamellae of Swollen Phospholipids [J]. J Mol Biol, 1965, 13(1): 238-252. Xia S, Xu S, Zhang X. Optimization in the Preparation of Coenzyme Q10 Nanoliposomes [J]. J Agric Food Chem, 2006, 54(17): 6358-6366. Liang M T, Davies N M, Toth I. Encapsulation of Lipopeptides within Liposomes: Effect of Number of Lipid Chains, Chain Length and Method of Liposome Preparation [J]. Int J Pharm, 2005, 301(1-2): 247-254. Brandl M, Bachmann D, Drechsler M, et al. Liposome Preparation by a New High Pressure Homogenizer Gaulin Micron Lab 40 [J]. Drug Dev Ind Pharm, 1990, 16(14): 2167-2191. Mayhew E, Lazo R, Vail W J, et al. Characterization of Liposomes Prepared Using a Microemulsifier [J]. Biochim Biophys Acta, 1984, 775(2): 169-174. Barnadas-Rodriguez R, Sabes M. Factors Involved in the Production of Liposomes with a High-Pressure Homogenizer [J]. Int J Pharm, 2001, 213(1-2): 175-186. Mabayo R T, Furuse M, Yang S I, et al. Medium-Chain Triacylglycerols Enhance Release of Cholecystokinin in Chicks [J]. J Nutr, 1992, 122: 1702-1705. Brouns F, van der Vusse G J. Utilization of Lipids during Exercise in Human Subjects: Metabolic and Dietary Constraints [J]. Brit J Nutr, 1998, 79: 117-128. Liu C M, Wang R L, Liu W, et al. Preparation Process of Medium Chain Fatty Acid Liposome and Its Characteristic Evaluation [J]. Food Science, 2007, 28(10): 143-146. (in Chinese) 刘成梅, 王瑞莲, 刘伟, 等. 中链脂肪酸脂质体的制备及其特性评价 [J]. 食品科学, 2007, 28(10): 143-146. Vuillemard J C. Recent Advances in the Large-Scale Production of Lipid Vesicles for Use in Food Products: Microfluidization [J]. J Microencapsul, 1991, 8(4): 547-562. Takahashi M, Inafuku K, Miyagi T, et al. Efficient Preparation of Liposomes Encapsulating Food Materials Using Lecithins by a Mechanochemical Method [J]. J Oleo Sci, 2007, 56(1): 35-42.
点击查看大图
计量
- 文章访问数: 7143
- HTML全文浏览量: 394
- PDF下载量: 744