超高压处理对海参自溶酶活性影响的研究

夏远景 刘志军 李宁 陈淑花 邓记松 刘学武 李志义

夏远景, 刘志军, 李宁, 陈淑花, 邓记松, 刘学武, 李志义. 超高压处理对海参自溶酶活性影响的研究[J]. 高压物理学报, 2009, 23(5): 377-384 . doi: 10.11858/gywlxb.2009.05.009
引用本文: 夏远景, 刘志军, 李宁, 陈淑花, 邓记松, 刘学武, 李志义. 超高压处理对海参自溶酶活性影响的研究[J]. 高压物理学报, 2009, 23(5): 377-384 . doi: 10.11858/gywlxb.2009.05.009
XIA Yuan-Jing, LIU Zhi-Jun, LI Ning, CHEN Shu-Hua, DENG Ji-Song, LIU Xue-Wu, LI Zhi-Yi. Effects of High Hydrostatic Pressure Treatment on Holothurians Autoenzyme Activity[J]. Chinese Journal of High Pressure Physics, 2009, 23(5): 377-384 . doi: 10.11858/gywlxb.2009.05.009
Citation: XIA Yuan-Jing, LIU Zhi-Jun, LI Ning, CHEN Shu-Hua, DENG Ji-Song, LIU Xue-Wu, LI Zhi-Yi. Effects of High Hydrostatic Pressure Treatment on Holothurians Autoenzyme Activity[J]. Chinese Journal of High Pressure Physics, 2009, 23(5): 377-384 . doi: 10.11858/gywlxb.2009.05.009

超高压处理对海参自溶酶活性影响的研究

doi: 10.11858/gywlxb.2009.05.009
详细信息
    通讯作者:

    李志义

Effects of High Hydrostatic Pressure Treatment on Holothurians Autoenzyme Activity

More Information
    Corresponding author: LI Zhi-Yi
  • 摘要: 海参的超高压处理与传统的处理方法相比有许多优越性,具有十分广阔的应用前景。研究了超高压处理过程中压力(0.1~550 MPa)、保压时间(0~30 min)、温度(24~62 ℃)及保压方式对海参自溶酶活性的影响。在室温、保压20 min的条件下,200 MPa左右较低压力下酶活性降低,相对残存活性为88.25%;250 MPa较高压力下自溶酶被激活,酶活性为106.77%;550 MPa高压下酶活性最低为29.81%。自溶酶活性随保压时间和温度的增加先上升后下降;保压方式对自溶酶活性的影响不大。同时利用误差反向传播神经网络(Back Propagation Neural Network,BP人工神经网络),模拟了超高压钝酶效果,与实验结果比较,平均相对误差为0.9%,可以获得较好的预测结果。研究结果表明,在一定的压力、保压时间和温度下,酶被激活,其活性上升;而在一定的压力、保压时间和温度下,酶被钝化,活性降低。对优化海参超高压钝酶工艺具有一定的参考价值。

     

  • Wu R P, Chen Y, Fang J R, et al. Studies on the Chemical Constituents from Sea Cucumber Mensamaria Intercedens Ⅳ Isolation, Propreties and Antitumor Activity of the Glycoprotein from Mensamaria Mntercedens [J]. Chinese Journal of Marine Drugs, 2000, 19(5): 4-6. (in Chinese)
    吴萍茹, 陈粤, 方金瑞, 等. 二色桌片参的化学成分研究Ⅳ二色桌片参糖蛋白的分离性质及抗肿瘤活性的研究 [J]. 中国海洋药物, 2000, 19(5): 4-6.
    Huang Y L, Zheng Z H, Su W J, et al. Studies on the Chemical Composition of Sea Cucumber Mensamaria Intercedens Ⅲ. Immunomodulative Effects of PMI-1 [J]. Marine Science Bulletin, 2001, 20 (1): 88-91. (in Chinese)
    黄益丽, 郑忠辉, 苏文金, 等. 二色桌片参的化学成分研究: Ⅲ二色桌片参多糖-1--岩藻聚糖的免疫调节作用 [J]. 海洋通报, 2001, 20 (1): 88-91.
    Maier M S, Roccatagliata A J, Kuriss A, et al. Two New Cytotoxic and Virucidal Trisulfated Triterpene Glycosides from the Antarctic Sea Cucumber Staurocucumis Liouvillei [J]. J Nat Prod, 2001, 64(6): 732-736.
    Rodriguez E, Gonzalez M, Caride B, et al. Nutritional Value of Holothuria Forskali Protein and Effects on Serum Lipid Profile in Rats [J]. J Physiol Biochem, 2000, 56(1): 39-43.
    Pacheco R G, Vicente C P, Zancan P, et al. Different Antithrombotic Mechanisms among Glycosaminoglycans Revealed with a New Fucosylated Chondroitin Sulfate from an Echinoderm [J]. Blood Coagul Fibrinolysis, 2000, 11(6): 563-573.
    Shen H, Chen J, Li H, et al. Progress of Studies on the Culture Techniques of Sea Cucumber Worldwide [J]. Hebei Fisheries, 2007, (6): 3-5. (in Chinese)
    沈辉, 陈静, 李华, 等. 国内外海参养殖技术研究概况 [J]. 河北渔业, 2007, (6): 3-5.
    Zhu B W, Han B. Characterization and Purification of Holothurians Autoenzyme [J]. Food and Fermentation Industries, 2004, 30(4): 132-137. (in Chinese)
    朱蓓薇, 韩冰. 海参自溶酶的分离纯化和部分性质研究 [J]. 食品与发酵工业, 2004, 30(4): 132-137.
    Anese M, Nicoli M C, Dallpaglio G, et al. Effect of High Pressure Treatments on Peroxidase and Polyphenoloxidase Activities [J]. J Food Biochemistry, 1995, 18(4): 285-293.
    Hendrickx M, Ludikhuyze L, Van den Broeck I, et al. Effects of High Pressure on Enzymes Related to Food Quality [J]. Trends in Food Science Technology, 1998, 9(5): 197-203.
    Gomes M R A, Ledward D A. Effect of High-Pressure Treatment on the Activity of Some Polyphenoloxidases [J]. Food Chemistry, 1996, 56(1): 1-5.
    Zeng Q M, Pan J, Xie H M, et al. Effect of High Pressure (HP) Treatments on Polyphenoloxidase (PPO) Activity in Pear Juices [J]. Chinese Journal of High Pressure Physics, 2004, 18(2): 144-148. (in Chinese)
    曾庆梅, 潘见, 谢慧明, 等. 超高压处理对多酚氧化酶活性的影响 [J]. 高压物理学报, 2004, 18(2): 144-148.
    Gao Y L, Wang Y X, Jiang H H. Optimization of Inactivation Conditions of High Hydrostatic Pressure Using Response Surface Methodology [J]. Scientia Agricultura Sinica, 2004, 37(10): 1544-1549. (in Chinese)
    高瑀珑, 王允祥, 江汉湖. 超高压杀菌条件的响应曲面法优化研究 [J]. 中国农业科学, 2004, 37(10): 1544-1549.
    Gao Y L, Wang Y X, Jiang H H. Optimization of Conditions to Sterilize Staphyloccus Aureus by Ultra-High Hydrostatic Pressure Using Response Surface Methodology [J]. Chinese Journal of High Pressure Physics, 2004, 18(3): 273-278. (in Chinese)
    高瑀珑, 王允祥, 江汉湖. 响应曲面法优化超高压杀灭金黄色葡萄球菌条件的研究 [J]. 高压物理学报, 2004, 18(3): 273-278.
    Huang X D, Pan J, Xie H M, et al. Effects of Bacteria Concentration on Sterilization of Escherichia Coli in High Pressure Process [J]. Food Science, 2007, 28(2): 169-173. (in Chinese)
    黄训端, 潘见, 谢慧明, 等. 菌液浓度对大肠杆菌超高压杀灭效果的影响 [J]. 食品科学, 2007, 28(2): 169-173.
    Otero L, Antonio D, Molina G. A Model for Real Thermal Control in High-Pressure Treatment of Foods [J]. Biotechnol Prog, 2002, 18(4): 904-908.
    Torrecilla J S, Otero L, Sanz P D. A Neural Network Approach for Thermal/Pressure Food Processing [J]. Journal of Food Engineering, 2004, 62(1): 89-95.
    Yu S, Wang H X, Ma Y J. Three-layer BP Network Model for Estimation of Casualties in an Earthquake [J]. Journal of Earthquake Engineering and Engineering Vibration, 2005, 25(6): 113-117. (in Chinese)
    于山, 王海霞, 马亚杰. 三层BP神经网络地震灾害人员伤亡预测模型 [J]. 地震工程与工程振动, 2005, 25(6): 113-117.
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出版历程
  • 收稿日期:  2008-11-30
  • 修回日期:  2009-05-26
  • 发布日期:  2009-10-15

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