Volume 21 Issue 1
Apr 2015
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XIE Hui-Ming, HUANG Xun-Duan, PAN Jian, ZENG Qing-Mei, WANG Hai-Xiang, JIANG Ye-Lei. Effect of High Pressure on Exoproteinase of Bacillus subtilis[J]. Chinese Journal of High Pressure Physics, 2007, 21(1): 95-102 . doi: 10.11858/gywlxb.2007.01.016
Citation: XIE Hui-Ming, HUANG Xun-Duan, PAN Jian, ZENG Qing-Mei, WANG Hai-Xiang, JIANG Ye-Lei. Effect of High Pressure on Exoproteinase of Bacillus subtilis[J]. Chinese Journal of High Pressure Physics, 2007, 21(1): 95-102 . doi: 10.11858/gywlxb.2007.01.016

Effect of High Pressure on Exoproteinase of Bacillus subtilis

doi: 10.11858/gywlxb.2007.01.016
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  • Corresponding author: XIE Hui-Ming
  • Received Date: 23 Dec 2005
  • Rev Recd Date: 06 Jul 2006
  • Publish Date: 05 Mar 2007
  • We studied the effect of high pressure process (HPP) on exoproteinase of Bacillus subtilis by using Box-Behnken response surface methodology. Experimental results showed that HPP could inhibit activity of exoproteinase significantly. In the course of treatment, pressure and temperature were key factors to passivate enzyme, and their interaction was notable. A model of exoproteinase passivation was established by design expert software, which determination coefficient was 0.974 7 and adjusted determination coefficient was 0.942 1. Analysis of variance implied that the model was significant. Therefore, the model could be used to analyze and predict. Additionally, the experiment indicated that exoproteinase activity correlated with lethal rate significantly (R=0.933 0). The exoproteinase could be regarded as an index to evaluate effect of HPP on microorganism.

     

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